anti heat shock cognate 70 kda protein  (Cell Signaling Technology Inc)

Journal: The FASEB Journal

Article Title: Myonectin deletion promotes adipose fat storage and reduces liver steatosis

doi: 10.1096/fj.201900520r

Figure Lengend Snippet: Figure 5. Myonectin deficiency alters lipid distribution in male mice fed an HFD. A–D) WT and myonectin-KO mice were euthanized at 39 wk of age after a 2–4-h unfed period in the morning. Unless otherwise specified, data in this figure are from the analyses of WT (n = 12) and KO (n = 16) male mice. The wet weight of liver (A), visceral (gonadal) fat pad (B), and subcutaneous (inguinal) fat pad (C) are presented along with representative images of H&E-stained liver sections from WT and KO mice (D). E, F) Quantification of TG (E) and cholesterol (F) levels in livers. G) Western blot analysis of Perilipin 2. A total of 2 separate gels were run, each with WT (n = 6) and KO (n = 8) samples. Perilipin 2 (PLIN2) levels were first normalized to HSC70 levels from the same gel and then normalized to the mean WT value. Data were then combined from both gels for analysis. H, I) Representative images of H&E-stained eWAT (H) and iWAT (I) sections from WT and KO male mice. J) Quantification of adipocyte size in eWAT sections (WT, n = 5; KO, n = 5). K) Quantification of adipocyte size in iWAT sections (WT, n = 4; KO, n = 5). *P , 0.05, **P , 0.01.

Article Snippet: Antibodies used: LPL (GTX101125; GeneTex, Irvine, CA, USA), heat shock cognate 71 kDa protein (Hsc70) (sc-7298; Santa Cruz Biotechnology, Dallas, TX, USA), perilipin 2 (PA1-16972; Thermo Fisher Scientific), mouse IgGHRP (7076; Cell Signaling Technology, Danvers, MA, USA), and rabbit IgG-HRP (7074; Cell Signaling Technology).

Techniques: Staining, Western Blot

Journal: The FASEB Journal

Article Title: Myonectin deletion promotes adipose fat storage and reduces liver steatosis

doi: 10.1096/fj.201900520r

Figure Lengend Snippet: Figure 7. Postprandial LPL activity in the s.c. (inguinal) fat depot is increased in myonectin-KO male mice fed an HFD. A) LPL activity in eWAT (WT, n = 12; KO, n = 16) and iWAT (WT, n = 12; KO, n = 15) from mice euthanized at 39 wk of age following a 2–4-h unfed period in the morning. B) LPL activity in eWAT and iWAT from mice (WT, n = 10; KO, n = 17) euthanized at 36 wk of age after 2-h refeeding following food removal overnight. C) LPL mRNA expression in the same eWAT (WT, n = 10; KO, n = 16) and iWAT (WT, n = 10; KO, n = 15) samples as in B. D, E) Western blot analysis of LPL in the same iWAT samples as in B. A total of 2 separate gels were run: 1 with 6 WT and 8 KO samples, and the other with 4 WT and 8 KO samples. LPL levels were first normalized to HSC70 levels from the same gel, then normalized to the mean WT value. Data were then combined from both gels for analysis (WT, n = 10; KO, n = 16) (E). Images from 1 gel are shown in D. ***P , 0.001.

Article Snippet: Antibodies used: LPL (GTX101125; GeneTex, Irvine, CA, USA), heat shock cognate 71 kDa protein (Hsc70) (sc-7298; Santa Cruz Biotechnology, Dallas, TX, USA), perilipin 2 (PA1-16972; Thermo Fisher Scientific), mouse IgGHRP (7076; Cell Signaling Technology, Danvers, MA, USA), and rabbit IgG-HRP (7074; Cell Signaling Technology).

Techniques: Activity Assay, Expressing, Western Blot